分子生物學技術, 為實驗室內可運用之基礎課程。主要課程內容是介紹近代分子生物學技術層面之發展，以及在實驗室常用的分子生物學技術之基本原理、方法。因此，本課程著重在實際一般實驗操作過程中可能會應用到的技術，操作過程可能會遇到的問題。

一、課程簡介:

1. Web
2. I.Enzymology of recombinant DNA-Restriction enzymes
3. II.Enzymology of recombinant DNA-Enzymes in DNA engineering
4. Isolation of nucleic acids, blotting and DNA sequencing
5. Plasmids and cloning strategies
6. Bacteriophage, cosmid, YAC
7. (Mid-term examination)
8. Primer design, PCR strategies, RACE
9. Hybridization probes and screening
10. Analyses of gene regulation
11. Construction of cDNA and genomic DNA libraries
12. Expression systems and purification
13. Transgenic animal and gene targeting
14. whole-mount in situ hybridization and other techniques


二、先修課程:

三、參考書目:1.Greene, J. J. and Rao, V. B. 1998 Recombinant DNA Principles and Methodologies.
2.Old, R. W. and Primrose, S. B. 2002 Principles of Gene Manipulation.